Destructive analysis at multiple in vitro and in vivo time points creates a costly critical bottleneck in development of engineered tissues and regenerative medicine approaches. Our laboratory research the applicability of a multimodal tissue diagnostic platform combining optical (Time-Resolved Fluorescence Spectroscopy (TRFS)) and ultrasound backscatter microscopy (UBM) technologies for label-free, real-time, non-destructive in vitro and in vivo analysis of composition, structure and function of engineered tissue constructs. A few examples of such studies conducted in collaboration with Dr. Athanasiou and Dr. Leach in Biomedical Engineering and Dr. Leigh Griffiths in the School of Veterinary Medicine are depicted below.
Chondrogenic differentiation of stem cells derived from human adipose tissue (ASC) on a 3-D matrix
TRFS and UBM of vascular patch material implanted in a porcine carotid arteriotomy model
The effects of specific treatments designed to alter the amount of collagen and collagen cross-links in engineered cartilage constructs can be detected based on intrinsic tissue fluorescence signatures.
B.Z. Fite, M. Decaris, Y.H. Sun, Y. Sun, A. Lam, C.K.L. Ho, J.K. Leach, L. Marcu. “Noninvasive Multimodal Evaluation of Bioengineered Cartilage Constructs Combining Time-Resolved Fluorescence and Ultrasound Imaging.” Tissue Eng Part C Methods, 17(4): 495-504, 2011. (Link) (PDF)
H. Fatakdawala, L.G. Griffiths, S. Humphrey, L. Marcu, “Time-resolved fluorescence spectroscopy and ultrasound backscatter microscopy for nondestructive evaluation of vascular grafts,” J. Biomed. Opt., 19(8), 080503 (2014). (Link)
Y. Sun, D. Responte, H. Xie, J. Liu, H. Fatakdawala, J. Hu, K.A. Athanasiou, and L. Marcu. “Nondestructive Evaluation of Tissue Engineered Articular Cartilage Using Time-Resolved Fluorescence Spectroscopy and Ultrasound Backscatter Microscopy” Tissue Engineering Part C: Methods. March 2012, 18(3): 215-226. (Link)