We research the development of imaging systems that make it possible for simultaneous detection and monitoring of compositional, morphological and functional features of biological tissues. Potential applications include in vivo imaging of biological models of human diseases such as cancer and atherosclerotic cardiovascular disease, non-destructive evaluation of bioengineered tissues/constructs, longitudinal studies during development and wound healing mechanisms. Multimodal imaging is widely considered to employ two or more imaging techniques in a single examination to allow acquisition of co-registered complementary data from tissue. We develop devices that integrate three imaging techniques namely: 1) fluorescence lifetime imaging (FLIm), which is sensitive to biochemical changes on tissue surface; 2) ultrasound backscatter microscopy (UBM) or intravascular ultrasound (IVUS) that allows evaluation of tissue microstructure and morphology; and 3) photoacoustic imaging (PAI), which is sensitive to changes in tissue vascularization.
Multimodal FLIm, UBM and PAI system
3D visualization of Hamster cheek pouch. Normal (top) and tumor (bottom)
Multimodal FLIm and IVUS system
H. Fatakdawala, S. Poti, F. Zhou, Y. Sun, J. Bec, J. Liu, D. Yankelevich, S. Tinling, R. Gandour-Edwards, D. Farwell, and L. Marcu, “Multimodal in vivo imaging of oral cancer using fluorescence lifetime, photoacoustic and ultrasound techniques,” Biomed. Opt. Express 4, 1724-1741 (2013). (Link)
Y. Sun, H. Xie, J. Liu, M. Lam, A. J. Chaudhari, F. Zhou, J. Bec, D. R. Yankelevich, A. Dobbie, S. L. Tinling, R. F. Gandour-Edwards, W. L. Monsky, D. Gregory Farwell and L. Marcu, “In vivo validation of a bimodal technique combining time-resolved fluorescence spectroscopy and ultrasonic backscatter microscopy for diagnosis of oral carcinoma,” Journal of Biomedical Optics 17(11), 116003-116003 (2012) (Link)
J. Bec, H. Xie, D. R. Yankelevich, F. Zhou, Y. Sun, N. Ghata, R. Aldredge and L. Marcu, “Design, construction, and validation of a rotary multifunctional intravascular diagnostic catheter combining multispectral fluorescence lifetime imaging and intravascular ultrasound,” Journal of Biomedical Optics 17(10), 106012-106011 (2012). (Link)