Multimodal Imaging Systems

We research the development of imaging systems that make it possible for simultaneous detection and monitoring of compositional, morphological and functional features of biological tissues. Potential applications include in vivo imaging of biological models of human diseases such as cancer and atherosclerotic cardiovascular disease, non-destructive evaluation of bioengineered tissues/constructs, longitudinal studies during development and wound healing mechanisms. Multimodal imaging is widely considered to employ two or more imaging techniques in a single examination to allow acquisition of co-registered complementary data from tissue. We develop devices that integrate three imaging techniques namely: 1) fluorescence lifetime imaging (FLIm), which is sensitive to biochemical changes on tissue surface; 2) ultrasound backscatter microscopy (UBM) or intravascular ultrasound (IVUS) that allows evaluation of tissue microstructure and morphology; and 3) photoacoustic imaging (PAI), which is sensitive to changes in tissue vascularization.

Multimodal FLIm, UBM and PAI system

The schematic diagram of the multimodal system combining FLIm, PAI and UBM subsystems. The multimodal tissue interrogation probe enabling the integration of three modalities consists of a ring ultrasonic transducer shared by the UBM and PAI sub-systems, an optical fiber which serves as excitation-collection pathway in the FLIm subsystem, and a ring of 16 optical fibers which serve as excitation pathway in the PAI subsystem.

The schematic diagram of the multimodal system combining FLIm, PAI and UBM subsystems. The multimodal tissue interrogation probe enabling the integration of three modalities consists of a ring ultrasonic transducer shared by the UBM and PAI sub-systems, an optical fiber which serves as excitation-collection pathway in the FLIm subsystem, and a ring of 16 optical fibers which serve as excitation pathway in the PAI subsystem. Probe imaging hamster cheek pouch is shown in the lower right inset.

 3D visualization of Hamster cheek pouch. Normal (top) and tumor (bottom)

                         a21R                                                                                                                                                      a3R

Multimodal FLIm and IVUS system

Bimodal intravascular balloon catheter

Bimodal intravascular balloon catheter

 
Related Publications

H. Fatakdawala, S. Poti, F. Zhou, Y. Sun, J. Bec, J. Liu, D. Yankelevich, S. Tinling, R. Gandour-Edwards, D. Farwell, and L. Marcu, “Multimodal in vivo imaging of oral cancer using fluorescence lifetime, photoacoustic and ultrasound techniques,” Biomed. Opt. Express  4, 1724-1741 (2013). (Link)

Y. Sun, H. Xie, J. Liu, M. Lam, A. J. Chaudhari, F. Zhou, J. Bec, D. R. Yankelevich, A. Dobbie, S. L. Tinling, R. F. Gandour-Edwards, W. L. Monsky, D. Gregory Farwell and L. Marcu, “In vivo validation of a bimodal technique combining time-resolved fluorescence spectroscopy and ultrasonic backscatter microscopy for diagnosis of oral carcinoma,” Journal of Biomedical Optics 17(11), 116003-116003 (2012) (Link)

J. Bec, H. Xie, D. R. Yankelevich, F. Zhou, Y. Sun, N. Ghata, R. Aldredge and L. Marcu, “Design, construction, and validation of a rotary multifunctional intravascular diagnostic catheter combining multispectral fluorescence lifetime imaging and intravascular ultrasound,” Journal of Biomedical Optics 17(10), 106012-106011 (2012). (Link)

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